Search results for "sample clean-up"

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Comparison of Two Solid-Phase Extraction (SPE) Methods for the Identification and Quantification of Porcine Retinal Protein Markers by LC-MS/MS

2018

Proper sample preparation protocols represent a critical step for liquid chromatography-mass spectrometry (LC-MS)-based proteomic study designs and influence the speed, performance and automation of high-throughput data acquisition. The main objective of this study was to compare two commercial solid-phase extraction (SPE)-based sample preparation protocols (comprising SOLA&micro

0301 basic medicineSwineGeneral Mathematicssample clean-upProteomicsMass spectrometry01 natural sciencesCatalysisRetinaArticlelcsh:ChemistryInorganic Chemistry03 medical and health scienceschemistry.chemical_compoundglaucoma animal modelZIPTIP® C18 pipette tipsTandem Mass SpectrometryLiquid chromatography–mass spectrometryTrifluoroacetic acidAnimalsSample preparationSolid phase extractionPhysical and Theoretical ChemistryEye Proteinslcsh:QH301-705.5Molecular BiologySpectroscopymass spectrometryZIPTIP<sup>®</sup> C18 pipette tipsReproducibilityChromatographyChemistryApplied Mathematics010401 analytical chemistryOrganic ChemistrySolid Phase ExtractionExtraction (chemistry)PipettebiomarkersGlaucomaGeneral Medicine0104 chemical sciencesComputer Science Applications030104 developmental biologylcsh:Biology (General)lcsh:QD1-999SOLAμTM HRP SPE spin platesPeptidesChromatography LiquidInternational Journal of Molecular Sciences
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